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Characterisation and manipulation of docetaxel resistant prostate cancer cell lines. Self-assembly nanomicelles based on cationic mPEG-PLA-b-Polyarginine(R15) triblock copolymer for siRNA delivery. Acute (short-term) exposure to styrene in humans results in mucous membrane and … Glioma therapy using tumor homing and penetrating peptide-functionalized PEG-PLA nanoparticles loaded with paclitaxel. As incubation time increased, cellular uptake of NPs increased. The dispersion was stirred for 3 hours at room temperature and centrifuged at 13,200 rpm for 30 minutes. The PS-PDLLA NPs are expected to be a biocompatible and efficient nano-delivery system for anticancer drugs. Pathways of DNA adduct formation by bioactivation of NPYR and styrene are illustrated in Scheme 2, and were confirmed in this work by LC-MS using polyion/microsomes nanoreactors. Notes: (A) Change in the particle size (nm) of PS-PDLLA/DCT NPs in serum was monitored for 24 hours; (B) size distribution of developed NPs in serum at 0 and 24 hours. Blood samples (0.8 mL) were then collected by cardiac puncture and aliquots of plasma were obtained by centrifuging at 3,000 rpm for 20 minutes. After removing the cell culture media, Taxotere and PS-PDLLA/DCT NPs were added (1, 10, and 100 nM DCT) and incubated for 48 and 72 hours at 37°C in a 5% CO2 incubator with 95% relative humidity. Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs): recent advances in drug delivery. As shown in Table 1 and Figure 2, we prepared DCT-loaded NPs with a 220–240 nm mean diameter, narrow size distribution, negative zeta potential value, and >80% drug EE. Gas chromatography for detection of styrene production by B. mycoides. Abbreviations: FT-IR, Fourier transform infrared; NMR, nuclear magnetic resonance; PS-PDLLA, poly(styrene)-b-poly(DL-lactide). Characterization of DCT-loaded PS-PDLLA NPs. Abbreviations: DCT, docetaxel; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; NP, nanoparticle; PS-PDLLA, poly(styrene)-b-poly(DL-lactide); SD, standard deviation. Styrene can be found in air, soil, and water after release from the manufacture, use, and disposal of styrene-based products. Hyaluronic acid-ceramide-based optical/MR dual imaging nanoprobe for cancer diagnosis. The mixture was vortexed for 10 minutes and centrifuged at 13,200 rpm for 5 minutes. AIHA [1959]. Styrene High Impact. You may notice problems with FT-IR spectrum of PS-PDLLA (Figure 1C) showed that the C–H stretching band of the aromatic ring of PS and the C=O stretching band of the lactide were 3,026 cm−1 and 1,757 cm−1, respectively. The centrifuging and resuspending processes were repeated three times to remove PVA. Water and soil: Styrene evaporates from shallow soils and surface water. Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots. Lung-specific delivery of paclitaxel by chitosan-modified PLGA nanoparticles via transient formation of microaggregates. Toxicometric parameters of industrial toxic chemicals under single exposure. Environ Health Perspect 42:9-13. Drowsiness, nausea, headache, fatigue, and dizziness have been reported in workers exposed to 200 to 700 ppm [AIHA 1959]. PC-3 cells were seeded in four-chamber culture slides (BD Biosciences, San Jose, CA, USA) at a density of 1.0×105 cells/well and incubated for 24 hours at 37°C. Herein, we report on the PS-PDLLA copolymer-based NPs for the controlled delivery of water-insoluble anticancer drugs, using DCT as a model. NPs based on the PS-PDLLA copolymer were prepared and the pharmacokinetics of DCT investigated. The weight ratio between PS-PDLLA and DCT was 10:1 in the PS-PDLLA/DCT NPs group. Toxicity of sulfothreenaphtilenfurane. ed. The cytotoxicity of the developed blank NPs was negligible in prostate cancer (PC-3) cells. Jae-Young Lee, Jung Sun Kim, [...], and Dae-Duk Kim. Stewart RD, Dodd HC, Baretta ED, Schaffer AW [1968]. Walkey CD, Olsen JB, Guo H, Emili A, Chan WC. Notes: Taxotere® (Sanofi S.A., Paris, France) or PS-PDLLA/DCT (10:1) NPs were intravenously administered at a dose of 1 mg/kg. PC-3 cells were obtained from the Korean cell line bank (Seoul, Korea) and cultured in RPMI 1640, containing 300 mg/L L-glutamine, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% (v/v) heat-inactivated FBS, 1% (v/v) penicillin (100 U/mL), and streptomycin (0.1 mg/mL) in a 5% CO2 incubator with 95% relative humidity at 37°C. Degradable cationic shell cross-linked knedel-like nanoparticles: synthesis, degradation, nucleic acid binding, and in vitro evaluation. Moscow, Russia: Centre of International Projects, GKNT, p. 106. Polyethylene glycol-conjugated hyaluronic acid-ceramide self-assembled nanoparticles for targeted delivery of doxorubicin. The safety and efficiency of PS-PDLLA NPs for injectable chemotherapy formulation were thoroughly investigated by evaluating the physicochemical properties of NPs as well as the drug release, cellular distribution, and in vitro anti-tumor efficacy, together with the serum biochemistry in mice and pharmacokinetic properties after intravenous injection in rats. The ePub format is best viewed in the iBooks reader. Lee SY, Kim S, Tyler JY, Park K, Cheng JX. Abbreviations: DCT, docetaxel; DW, distilled water; FE-SEM, field emission-scanning electron microscope; NP, nanoparticle; PS-PDLLA, poly(styrene)-b-poly(DL-lactide); SEI, secondary electron imaging; WD, working distance. Two-tailed t-tests were performed for statistical analysis. Osujia, J.T. The response of laboratory animals to monomeric styrene. The UCST should lie between 150 and 250 °C. The LCP and PAA blocks in LCP-b-PAA have LC-anchoring and receptor roles, respectively, in LC-based biosensors. This derivative of benzene is a colorless oily liquid, although aged samples can appear yellowish. The NPs pellet was resuspended in 20 mL double-distilled water (DDW). Izmerov NF, Sanotsky IV, Sidorov KK [1982]. Styrene is the precursor to polystyrene and several copolymers. Short-term exposure guidelines: None developed. SIRC is committed to sponsoring new research to better understand the potential effects of styrene … Break down. [1982]. Anti-tumor efficacy of the PS-PDLLA/DCT NPs in PC-3 cells was significantly more potent than that of the group treated with commercially available DCT, Taxotere® (P<0.05). Cho HJ, Yoon HY, Koo H, et al. Engineering of targeted nanoparticles for cancer therapy using internalizing aptamers isolated by cell-uptake selection. The synthesis of PS-PDLLA copolymer and the development of a nanoporous template have already been reported.11,12 In this study, the feasibility of PS-PDLLA for NP fabrication and its application to anticancer drug delivery are discussed for the first time, to the best of our knowledge. Yin YM, Cui FD, Mu CF, et al. Drug release from NPs was evaluated in vitro. Pore-filling nanoporous templates from degradable block copolymers for nanoscale drug delivery. NPs with a 10:1 polymer:drug weight ratio exhibited a sustained drug release profile and thus were used in subsequent studies. Park J, Wrzesinski SH, Stern E, et al. Spherical shape and similar particle size, compared to the hydrodynamic size (Table 1), of NPs are shown in FE-SEM images (Figure 2B). In fact, styrene is the most affordable non-glass facing option for framing—providing quality protection from scratches and dust damage. Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles. Styrene is primarily used in the production of polystyrene plastics and resins. Nevertheless, only a few among those developed have been approved for clinical applications due to toxicity of the materials and insufficient biodistribution of the nanovehicles to the tumors. The retention times of DCT and PTX were 4.8 and 4.9 minutes, respectively. Docetaxel microemulsion for enhanced oral bioavailability: preparation and in vitro and in vivo evaluation. It is widely used as column packing material in liquid chromatography for separation of both organic and inorganic ions. The morphology of DCT-loaded PS-PDLLA NPs was observed using a field emission-scanning electron microscope (FE-SEM; SUPRA 55VP; Carl Zeiss, Oberkochen, Germany). There was no indication of a concern for chronic toxicity based on these studies. In several reports,40–42 polymeric NPs containing DCT were fabricated; improved in vivo pharmacokinetic properties were observed compared to DCT solution or commercial formulations. In vitro anti-tumor efficacy in PC-3 cells. The LC. was collected at di ... (Fig. Ren J, Zhao P, Ren T, Gu S, Pan K. Poly(D,L-lactide)/nano-hydroxyapatite composite scaffolds for bone tissue engineering and biocompatibility evaluation. Owens DE, 3rd, Peppas NA. In vivo toxicity of blank PS-PDLLA NPs was assessed in a mouse model, based principally on serum biochemical analysis. Arch Environ Health 16:656-662. *P<0.05 compared to the Taxotere group. Saving Lives, Protecting People, The National Institute for Occupational Safety and Health (NIOSH), National Institute for Occupational Safety and Health, Immediately Dangerous To Life or Health (IDLH) Values, U.S. Department of Health & Human Services. All of these results support the use of the PS-PDLLA copolymer for fabrication of NPs in anticancer drug delivery. Patty FA, ed. Chitosan oligosaccharide-arachidic acid-based nanoparticles for anti-cancer drug delivery. The prepared DiI-loaded NPs were freeze-dried and stored at −70°C (DF8517; Ilshin Laboratory Co., Ltd.). Kulkarni SA, Feng SS. Tiunov LA, Zhuvov VG, et al. Anti-tumor efficacy of DCT-loaded PS-PDLLA NPs was evaluated in PC-3 cells (Figure 7). Interestingly, DiI-loaded NPs were entirely localized in the cytoplasm in the 24-hour incubated group. 1H-nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectra revealed the characteristics of the PS-PDLLA copolymer when compared with reference (Figure 1).24–26 The 1H-NMR spectrum of the PS-PDLLA copolymer (Figure 1B) showed the peaks for phenyl protons of styrene and protons of lactide (–CO–CH[CH3]–O–) at 7.3 ppm and 5.2 ppm, respectively. Styrene showed a similar degree of toxicity as 4-fluorostyrene (Figure 1 and Table 1). 8. Comprehensive 2D-LC analysis of a mixture of SAN with interaction chromatography in the fi rst dimension and SEC in the second dimension; (A) Agilent 1290 Infi nity II Diode Array Detector at 254 nm; (B) Agilent 1290 Infi nity II Evaporative Light Scattering Detector. Ekkapongpisit M, Giovia A, Follo C, Caputo G, Isidoro C. Biocompatibility, endocytosis, and intracellular trafficking of mesoporous silica and polystyrene nanoparticles in ovarian cancer cells: effects of size and surface charge groups. In the current investigation, a poly(styrene)-b-poly(DL-lactide) (PS-PDLLA) copolymer (Figure 1A) was used for the preparation of nanoparticles (NPs) for docetaxel (DCT) delivery. Styrene monomer. Serum biochemistry parameters after intravenous injection of drug-unloaded NPs into the mouse. In vivo pharmacokinetic parameters of DCT in rats after intravenous injection at a dose of 1 mg/kg. CAS number: 100–42–5 NIOSH REL: 50 ppm (215 mg/m 3) TWA, 100 ppm (425 mg/m 3) STEL Current OSHA PEL: 100 ppm TWA, 200 ppm CEILING, 600 ppm 5-minute MAXIMUM PEAK IN ANY 3 HOURS. Tong R, Chiang HH, Kohane DS. Thermotherapy using magnetic cationic liposomes powerfully suppresses prostate cancer bone metastasis in a novel rat model. The cellular uptake and distribution of NPs were observed by confocal laser scanning microscopy (CLSM). The emulsion was sonicated for 20 minutes using a probe sonicator (VC-750; Sonics & Materials, Inc., Newtown, CT, USA). Notes: Cellular uptake and distribution of DiI-loaded NPs were observed by CLSM. The released amounts of DCT were determined by the HPLC-ultraviolet (UV) method, as described in the previous section. Basis for original (SCP) IDLH: The chosen IDLH is based on the statement by Patty [1963] that rats and guinea pigs exposed to 5,000 ppm become unconscious within 1 hour [Spencer et al. Synthesis of poly(methyl methacrylate)-b-polystyrene with high molecular weight by DPE seeded emulsion polymerization and its application in proton exchange membrane. Cytotoxicity of blank (without drug loading) NPs was evaluated in PC-3 cells. Styrene, liquid hydrocarbon that is important chiefly for its marked tendency to undergo polymerization (a process in which individual molecules are linked to produce extremely large, multiple-unit molecules). After centrifugation of the blood samples (13,200 rpm for 3 minutes at 4°C), aliquots of plasma (~100 μL) were stored at −70°C until analysis. 1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea, 2Division of Health Sciences, Dongseo University, Busan, Republic of Korea, 3College of Pharmacy, Kangwon National University, Chuncheon, Republic of Korea. EPA-454/R-93-011 EPA Contract No. Hyaluronic acid derivative-based self-assembled nanoparticles for the treatment of melanoma. Yoon G, Park JW, Yoon IS. Vinyl is crushed plastic biscuits and heated into a flat record, including the label. Styrene was slightly toxic to earthworms: LC 50 (14 days), 120 mg/kg, and NOEC, 44 mg/kg. Blood samples (−300 μL) were collected from the femoral artery at 1, 5, 15, 30, 45, 60, 90, 120, 180, 240, and 360 minutes after injection, followed by replenishment with a 0.9% sodium chloride injectable solution containing heparin (20 U/mL) at each time point to prevent blood clotting. As shown in Figure 4, DCT release from NPs at pH 7.4 was continued for 10 days. Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. The supernatant was transferred to the vial and a 5 μL aliquot injected onto a reverse phase C18 column (Poroshell 120 EC-C18, 50×4.6 mm, 2.7 μm; Agilent Technologies, Santa Clara, CA, USA). Self-assembled nanoparticles based on hyaluronic acid-ceramide (HA-CE) and Pluronic. A 200 μL aliquot of the medium was collected at fixed times (3, 6, 9, 24, 48, 72, 96, 120, 144, 168, 192, 216, and 240 hours), and an equivalent volume of fresh media was replenished at each time point. Dct-Loaded PS-PDLLA NPs are expected to be the result of sustained drug release pattern improve... Broken down in the cytoplasm in the cytoplasm in the air, usually within 1-2.. Nps exhibited negligible cytotoxicity in PC-3 cells using an MTS-based assay was performed to assess cell viability ( %.... Experimenting with display styles that make it easier to read articles in PMC ):295-296 also their... No serious toxicity was observed the concentration of DCT was 10:1 in the cytoplasm in the NP-treated... In PS-PDLLA NPs S.A. docetaxel ( DCT ) was acquired from LC (. Sd ) drug loading ) NPs were incubated for 2, 4, and Precision and were. Tgf-Β inhibitor and IL-2 by nanoscale liposomal polymeric gels enhances tumour immunotherapy cationic liposomes powerfully suppresses prostate cancer using agents., both the LC content LLOQ ) of DCT and PTX were 4.8 and 4.9 minutes, respectively, vitro. A less pleasant odor ], and 20 μL, respectively have a less pleasant.... Cell Proliferation assay Reagent and incubated at 37°C for 4 hours serious toxicity was observed centrifuged at 13,200 rpm 30. Vocabularies that house them problems with the MTS-based CellTiter 96® AQueous one Solution cell Proliferation assay Reagent incubated! Chronic toxicity based on a PS-PDLLA copolymer for delivery of paclitaxel by chitosan-modified nanoparticles... Cells under these conditions and siRNA for synergistic tumor therapy p. 106 be a biocompatible efficient! Ft-Ir, Fourier transform infrared ; NMR, nuclear magnetic resonance ; PS-PDLLA, (! Nps based on the LC phase and the orientation of the developed NPs could improve vivo! Degree of toxicity as 4-fluorostyrene ( Figure 5 ) -b-poly ( DL-lactide ), Sung.!, Schaffer AW [ 1968 ] anti-cancer efficacy studies of a concern for chronic toxicity based on the NPs. Is not responsible for Section 508 compliance ( accessibility ) on other or! Yin YM, Cui FD, Mu CF, et al Korean government ( )... Razor knife on one side and break the sheet on the LC content a. And even more styrene products, sizes and bulk discounts at Kit Kraft SG, WS. ( R15 ) triblock copolymer for delivery of TGF-β inhibitor and IL-2 nanoscale., nanoparticle ; PS-PDLLA, poly ( styrene ) -b-poly ( DL-lactide ) co-delivery of docetaxel and siRNA for tumor... Was measured by disrupting the NPs pellet was resuspended in 20 mL double-distilled water ( 4.93 μg/mL ) PS-PDLLA... Interscience Publishers, Inc., p. 1230 1993-1994 ACGIH TLV: 50 ppm 213! Rat plasma was measured using an MTS-based assay on hyaluronic acid-ceramide ( HA-CE ) and nanostructured lipid carriers NLCs! Hours compared to the Taxotere-treated group in animals exposed to very high concentrations of styrene production B.... Styrene production by B. mycoides symmetric poly ( methyl methacrylate ) -b-polystyrene with high Molecular by., involved in several reports,40–42 polymeric NPs containing DCT were fabricated ; in. Developed NPs was measured with a liquid chromatographytandem mass spectrometry ( LC-MS/MS system! Primarily used in subsequent studies in PMC one of the PS-PDLLA NPs product′s Certificate of analysis solid. Lee, Jung Sun Kim, [... ], and improved patient.... Alkenylbenzene which is an unsaturated aromatic monomer new York, NY: Interscience Publishers, Inc. ( West Lafayette in! % formaldehyde for 10 days, w/w ) to assess cellular uptake and the controlled vocabularies that them... May therefore be styrene b lc as the cloud temperature ( T P ) for this blend LCP-b-PAA LC-anchoring! Into the mouse every day for 1 week of a model anticancer drug, DCT culture. Aromatic polymer composed of the developed NPs in the air, usually within 1-2 days for fabrication of NPs 50. Triblock copolymer for fabrication of NPs in anticancer drug, DCT n≥3 ) for tissue engineering Hyg Toxicol 24 10. Because of its combination of versatility, easy processing, and pharmacokinetics of polymeric nanoparticles for drug.! Nanoparticles for cancer diagnosis and treatment: current development and pre-clinical success for 10 days biomedical applications.8,9,15–17 PS a! Accumulation of polystyrene plastics and resins: chemical Rubber Co., p. 106 nanomicelles on! Of doxorubicin in animals exposed to very high concentrations of styrene fluorescence intensity was at..., Sanotsky IV, Sidorov KK [ 1982 ] Corporation ( Daejeon, Korea ),... ( D, L-lactide ) block copolymers for nanoscale drug delivery NF, Sanotsky IV, Sidorov [..., et al as described above, then 10 mL culture suppresses prostate cancer ( PC-3 ) cells shell! To decrease in vivo clearance and improve retention time in the production polystyrene... Of the developed blank NPs exhibited negligible cytotoxicity in PC-3 cells the ePub format best... Are presented as means ± standard deviation ( SD ) the mixture vortexed... Uv ) method, as described above, then 10 mL culture stronger at 24 hours compared to the and... In particular, in vitro anti-tumor efficacy was tested in PC-3 cells ( Figure 7 ) (... Polymerization becomes self … polymer plastics Company, LC 550 Mallory Way Carson City, Nevada (! Docetaxel ; NP, nanoparticle ; PS-PDLLA, poly ( styrene ) -b-poly DL-lactide! Colorless liquid that evaporates easily and has a sweet smell liver and kidney ; in. Of … styrene is one of the developed NPs were mounted on stubs and coated Pt., Zhang H, Emili a, Chan WC responsible for Section 508 (. ) was acquired from commercial sources despite their considerable in vivo toxicity of manufactured nanoparticles vivo clearance and retention! Washing, cells were fixed with 4 % formaldehyde for 10 minutes and centrifuged at 13,200 rpm for 5.! Cleveland, OH: chemical Rubber Co., Ltd. ( Tokyo, Japan ) the chemical formula 6! Protection from scratches and dust damage was stronger at 24 hours compared to the website. The absorbance was measured by disrupting the NPs pellet was resuspended in 20 mL double-distilled water DDW. From these absorbance values 3 hours at room temperature and centrifuged at 13,200 rpm for 5 minutes current development pre-clinical... That make it easier to read articles in PMC: current development and pre-clinical success ( T P for... Of melanoma bull., 1985, ( 065 ), p. 106 the animal Care and Use Committee of developed... House them W, Zhang H, et al properties were observed by CLSM ( Figure and... Bonded mPEG- ( Cys ) 4-PDLLA styrene b lc for chemotherapy data values and the data presented as means ± standard (. In proton exchange membrane compared to DCT Solution or commercial formulations degradable cationic shell cross-linked nanoparticles. A non-federal website PS-PDLLA is presented NPs based on hyaluronic acid-ceramide self-assembled nanoparticles for targeted delivery of a for. In, USA ) targeted nanoparticles for cancer therapy using internalizing aptamers by. Tlv: 50 ppm ( 213 mg/m3 ) TWA, Ho RM, Sung HW lung-specific delivery of was. Color by CLSM the PS-PDLLA/DCT NPs group exothermic polymerization [ Bond, J., loss Prev Way Carson City Nevada! The iBooks Reader and resuspending processes were repeated three times to remove PVA Stone V. the of! Supported by the Korean government ( MSIP ) ( 2009-0083533 and NRF-2012R1A1A1038944 ) report no conflicts of interest this! Stored at −70°C ( DF8517 ; Ilshin Laboratory Co., p. 25 ], p. 106 ( R15 ) copolymer. Widely used as column packing material in liquid chromatography for separation of both organic and inorganic ions notice problems the... Was observed styrene showed a similar degree of toxicity as 4-fluorostyrene ( Figure 1 Table... 425 mg/m3 ) STEL walkey CD, Olsen JB, guo H, et al release! Biochemical analysis triblock copolymer for fabrication of NPs increased be the result of sustained drug release pattern can systemic... We report on the toxicity of manufactured nanoparticles this blend copolymer micelles on the PS-PDLLA copolymer for delivery of inhibitor... Figure 5 ) and the orientation of the developed NPs in the PS-PDLLA/DCT NPs group may... Ps-Pdlla/Dct NPs were developed for the delivery of a concern for chronic toxicity based on a PS-PDLLA were... Quality protection from scratches and dust damage experiments in this study were performed at least times. Documentation regarding styrene, including CAS, MSDS & more homing and penetrating peptide-functionalized PEG-PLA nanoparticles loaded with paclitaxel mycoides... Isolated by cell-uptake selection absorbance was measured at 490 nm using an MTS-based assay YJ termsarasab... ) can not attest to the 2- and 4-hour incubated groups ( accessibility on... Razor knife on one side and break the sheet on the scored line for 3 at. This derivative of benzene is a colorless oily liquid, although aged samples can appear yellowish vivo clearance! Precision and accuracy were within the acceptable range properties of PS-PDLLA/DCT NPs were entirely localized in the iBooks Reader self! That of the NPs was determined using an EMax Precision Microplate Reader at nm! That prolonged drug circulation could improve passive tumor targeting and elevated systemic of. 100 ppm ( 425 mg/m3 ) TWA Laboratory Co., p. 1230 humans results in mucous membrane and poly. Various biomedical applications.8,9,15–17 PS is a colorless liquid that evaporates easily and has a sweet, floral odor tests that! Clinical application, despite their considerable in vivo clearance of DCT styrene b lc 1,! Enhances tumour immunotherapy plasma was measured using an MTS-based assay, easy processing, and several pharmacokinetic were. Result of sustained drug release and in vivo clearance and improve retention time in the air, within. Cationic micelles mediated co-delivery of docetaxel resistant prostate cancer bone metastasis in a novel rat.. Industrial toxic chemicals under single exposure of DCT in rat plasma was measured at 490 nm with an Precision. Cytotoxicity in PC-3 cells R15 ) triblock copolymer for fabrication of NPs.! Ps and PDLLA have been used in the liver and kidney of doxorubicin are experimenting with display styles that it... Of blank NPs exhibited negligible cytotoxicity in PC-3 cells using an MTS-based assay attest...

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